دانلود گزارش کارورزی و کار آموزی

چکیده : 

A series of melphalan-O-carboxymethyl chitosan (Mel-OCM-chitosan) conjugates with different spacerswere prepared and structurally characterized. All conjugates showed satisfactory water-solubility (160-217 times of Mel solubility). In vitro drug release behaviors by both chemical and enzymatic hydrolysiswere investigated. The prodrugs released Mel rapidly within papain and lysosomal enzymes of about40–75%, while released only about 4–5% in buffer and plasma, which suggested that the conjugates havegood plasma stability and the hydrolysis in both papain and lysosomes occurs mostly via enzymolysis.It was found that the spacers have important effect on the drug content, water solubility, drug releaseproperties and cytotoxicity of Mel-OCM-chitosan conjugates. Cytotoxicity studies by MTT assay demon-strated that these conjugates had 52–70% of cytotoxicity against RPMI8226 cells in vitro as comparedwith free Mel, indicating the conjugates did not lose anti-cancer activity of Mel. Overall these studiesindicated Mel-OCM-chitosan conjugates as potential prodrugs for cancer treatment